LNPEP Kit ELISA

N° du produit ABIN5656394

Détail du produit LNPEP Kit ELISA

Antigène: LNPEP (Leucyl/cystinyl Aminopeptidase (LNPEP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Leucyl/Cystinyl Aminopeptidase (LNPEP). No significant cross-reactivity or interference between Leucyl/Cystinyl Aminopeptidase (LNPEP) and analogues was observed.

Sensibilité: 0.062 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucyl/Cystinyl Aminopeptidase (LNPEP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucyl/Cystinyl Aminopeptidase (LNPEP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucyl/Cystinyl Aminopeptidase (LNPEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leucyl/Cystinyl Aminopeptidase (LNPEP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucyl/Cystinyl Aminopeptidase (LNPEP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucyl/Cystinyl Aminopeptidase (LNPEP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LNPEP (Leucyl/cystinyl Aminopeptidase (LNPEP))

Autre désignation: Leucyl/Cystinyl Aminopeptidase (LNPEP Produits)

Synonymes: fb73h12 Kit ELISA, wu:fb73h12 Kit ELISA, zgc:66103 Kit ELISA, 2010309L07Rik Kit ELISA, 4732490P18Rik Kit ELISA, CAP Kit ELISA, IRAP Kit ELISA, PLAP Kit ELISA, gp160 Kit ELISA, vp165 Kit ELISA, P-LAP Kit ELISA, Vp165 Kit ELISA, LNPEP Kit ELISA, leucyl and cystinyl aminopeptidase Kit ELISA, leucyl/cystinyl aminopeptidase L homeolog Kit ELISA, leucyl/cystinyl aminopeptidase Kit ELISA, LNPEP Kit ELISA, lnpep.L Kit ELISA, lnpep Kit ELISA, Lnpep Kit ELISA

Sujet:

Gene Name: Leucyl/Cystinyl Aminopeptidase

Gene Aliases: CAP, PLAP, OTASE, IRAP, Oxytocinase, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase, Placental leucine aminopeptidase

Pathways: Regulation of long-term Neuronal Synaptic Plasticity