Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

LASP1 Kit ELISA

LASP1 Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5656420
  • Antigène Voir toutes LASP1 Kits ELISA
    LASP1 (LIM and SH3 Protein 1 (LASP1))
    Reactivité
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of LIM And SH3 Protein 1 (LASP1). No significant cross-reactivity or interference between LIM And SH3 Protein 1 (LASP1) and analogues was observed.
    Sensibilité
    0.065 ng/mL
    Featured
    Discover our best selling LASP1 Kit ELISA
    Top Product
    Discover our top product LASP1 Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM And SH3 Protein 1 (LASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM And SH3 Protein 1 (LASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM And SH3 Protein 1 (LASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LIM And SH3 Protein 1 (LASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LIM And SH3 Protein 1 (LASP1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LIM And SH3 Protein 1 (LASP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes LASP1 Kits ELISA
    LASP1 (LIM and SH3 Protein 1 (LASP1))
    Autre désignation
    LIM And SH3 Protein 1 (LASP1 Produits)
    Synonymes
    lasp1 Kit ELISA, MGC53336 Kit ELISA, LASP1 Kit ELISA, MGC89667 Kit ELISA, Lasp-1 Kit ELISA, MLN50 Kit ELISA, AA408629 Kit ELISA, Def-4 Kit ELISA, SH3P6 Kit ELISA, Tg(Col1a1-lacZ)1Ngma Kit ELISA, fb92f05 Kit ELISA, wu:fb92f05 Kit ELISA, zgc:77542 Kit ELISA, lasp-1 Kit ELISA, mln50 Kit ELISA, LIM and SH3 protein 1 Kit ELISA, LIM and SH3 protein 1 L homeolog Kit ELISA, lasp1 Kit ELISA, lasp1.L Kit ELISA, LASP1 Kit ELISA, Lasp1 Kit ELISA
    Sujet

    Gene Name: LIM And SH3 Protein 1

    Gene Aliases: MLN50, Metastatic lymph node gene 50 protein

    ID gène
    3927
    UniProt
    Q14847
Vous êtes ici:
Support technique