LIM Domain Binding 1 Protein Kit ELISA

N° du produit ABIN5656421

Détail du produit LIM Domain Binding 1 Protein Kit ELISA

Antigène: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of LIM Domain Binding Protein 1 (LDB1). No significant cross-reactivity or interference between LIM Domain Binding Protein 1 (LDB1) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM Domain Binding Protein 1 (LDB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM Domain Binding Protein 1 (LDB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM Domain Binding Protein 1 (LDB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LIM Domain Binding Protein 1 (LDB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LIM Domain Binding Protein 1 (LDB1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LIM Domain Binding Protein 1 (LDB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Autre désignation: LIM Domain Binding Protein 1 (LDB1 Produits)

Synonymes: CLIM2 Kit ELISA, NLI Kit ELISA, chi Kit ELISA, clim2 Kit ELISA, nli Kit ELISA, xldb1 Kit ELISA, ldb1 Kit ELISA, LDB1 Kit ELISA, ldb4 Kit ELISA, ldb4b Kit ELISA, LIM domain binding 1 Kit ELISA, LIM domain binding 1 L homeolog Kit ELISA, LIM-domain binding 1b Kit ELISA, LIM domain binding protein 1 Kit ELISA, LIM domain binding 1a Kit ELISA, Ldb1 Kit ELISA, LDB1 Kit ELISA, ldb1.L Kit ELISA, ldb1b Kit ELISA, xldb1 Kit ELISA, ldb1a Kit ELISA

Sujet:

Gene Name: LIM Domain Binding Protein 1

Gene Aliases: CLIM2, NLI, Carboxy Terminal LIM Domain Protein 2, LIM domain-binding factor CLIM2, Nuclear LIM interactor

ID gène: 8861

UniProt: Q86U70

Pathways: Stem Cell Maintenance, Chromatin Binding