LRP1 Kit ELISA
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- Antigène Voir toutes LRP1 Kits ELISA
- LRP1 (Low Density Lipoprotein Receptor-Related Protein 1 (LRP1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 62.5 pg/mL - 4000 pg/mL
- Seuil minimal de détection
- 62.5 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Low Density Lipoprotein Receptor Related Protein 1 (LRP1). No significant cross-reactivity or interference between Low Density Lipoprotein Receptor Related Protein 1 (LRP1) and analogues was observed.
- Sensibilité
- 28.4 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Related Protein 1 (LRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Related Protein 1 (LRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Density Lipoprotein Receptor Related Protein 1 (LRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Low Density Lipoprotein Receptor Related Protein 1 (LRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 1 (LRP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 1 (LRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes LRP1 Kits ELISA
- LRP1 (Low Density Lipoprotein Receptor-Related Protein 1 (LRP1))
- Autre désignation
- Low Density Lipoprotein Receptor Related Protein 1 (LRP1 Produits)
- Synonymes
- A2MR Kit ELISA, APOER Kit ELISA, APR Kit ELISA, CD91 Kit ELISA, IGFBP3R Kit ELISA, LRP Kit ELISA, LRP1A Kit ELISA, TGFBR5 Kit ELISA, A2mr Kit ELISA, AI316852 Kit ELISA, Lrp Kit ELISA, LRP-1 Kit ELISA, LDL receptor related protein 1 Kit ELISA, prolow-density lipoprotein receptor-related protein 1 Kit ELISA, low density lipoprotein receptor-related protein 1 Kit ELISA, LRP1 Kit ELISA, Lrp1 Kit ELISA, LOC100414789 Kit ELISA
- Sujet
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Gene Name: Low Density Lipoprotein Receptor Related Protein 1
Gene Aliases: CD91, LRP, A2MR, APOER, APR, TGFBR5, Alpha-2-Macroglobulin Receptor, Prolow-density lipoprotein receptor-related protein 1, Apolipoprotein E receptor
- ID gène
- 4035
- UniProt
- Q07954
- Pathways
- Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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