LHB Kit ELISA

N° du produit ABIN5656514

Détail du produit LHB Kit ELISA

Antigène: LHB (Luteinizing Hormone beta Polypeptide (LHB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 246.91 pg/mL - 20000 pg/mL

Seuil minimal de détection: 246.91 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone Beta Polypeptide (LHb). No significant cross-reactivity or interference between Luteinizing Hormone Beta Polypeptide (LHb) and analogues was observed.

Sensibilité: 89.2 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 h

Plaque: Pre-coated

Protocole: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Luteinizing Hormone Beta Polypeptide (LHb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone Beta Polypeptide (LHb) and unlabeled Luteinizing Hormone Beta Polypeptide (LHb) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone Beta Polypeptide (LHb). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone Beta Polypeptide (LHb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Luteinizing Hormone Beta Polypeptide (LHb) in the sample.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone Beta Polypeptide (LHb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone Beta Polypeptide (LHb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LHB (Luteinizing Hormone beta Polypeptide (LHB))

Autre désignation: Luteinizing Hormone Beta Polypeptide (LHB Produits)

Synonymes: CGB4 Kit ELISA, LSH-B Kit ELISA, hLHB Kit ELISA, LH Kit ELISA, LH-B Kit ELISA, LSH-beta Kit ELISA, cgb4 Kit ELISA, hlhb Kit ELISA, xlhb Kit ELISA, lhb-A Kit ELISA, lsh-b Kit ELISA, LH/CG-beta Kit ELISA, LHB Kit ELISA, LSH-B/CG-B Kit ELISA, Lutropin beta chain Kit ELISA, lhb1 Kit ELISA, lhbeta1 Kit ELISA, zgc:92312 Kit ELISA, lhb Kit ELISA, luteinizing hormone beta polypeptide Kit ELISA, luteinizing hormone beta Kit ELISA, lutropin subunit beta-like Kit ELISA, luteinizing hormone, beta polypeptide Kit ELISA, luteinizing hormone beta polypeptide L homeolog Kit ELISA, LHB Kit ELISA, Lhb Kit ELISA, lhb Kit ELISA, LOC106838274 Kit ELISA, lhb.L Kit ELISA

Sujet:

Gene Name: Luteinizing Hormone Beta Polypeptide

Gene Aliases: LH-B, CGB4, LSH-B, hLHB, Lutropin Subunit Beta, Interstitial Cell Stimulating Hormone,Beta Chain

ID gène: 3972

UniProt: P01229

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, C21-Steroid Hormone Metabolic Process