LCP1 Kit ELISA

N° du produit ABIN5656524

Détail du produit LCP1 Kit ELISA

Antigène: LCP1 (Lymphocyte Cytosolic Protein 1 (LCP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Lymphocyte Cytosolic Protein 1 (LCP1). No significant cross-reactivity or interference between Lymphocyte Cytosolic Protein 1 (LCP1) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Cytosolic Protein 1 (LCP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Cytosolic Protein 1 (LCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Cytosolic Protein 1 (LCP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Cytosolic Protein 1 (LCP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphocyte Cytosolic Protein 1 (LCP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphocyte Cytosolic Protein 1 (LCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LCP1 (Lymphocyte Cytosolic Protein 1 (LCP1))

Autre désignation: Lymphocyte Cytosolic Protein 1 (LCP1 Produits)

Synonymes: CP64 Kit ELISA, L-PLASTIN Kit ELISA, LC64P Kit ELISA, LPL Kit ELISA, PLS2 Kit ELISA, cb245 Kit ELISA, fj24b12 Kit ELISA, wu:fj24b12 Kit ELISA, cp64 Kit ELISA, l-plastin Kit ELISA, lc64p Kit ELISA, lpl Kit ELISA, pls2 Kit ELISA, plastin-2 Kit ELISA, AW536232 Kit ELISA, D14Ertd310e Kit ELISA, LCP-1 Kit ELISA, Pls2 Kit ELISA, pp65 Kit ELISA, lymphocyte cytosolic protein 1 Kit ELISA, lymphocyte cytosolic protein 1 (L-plastin) Kit ELISA, lymphocyte cytosolic protein 1 (L-plastin) S homeolog Kit ELISA, lower matrix protein; involved in immune regulation Kit ELISA, LCP1 Kit ELISA, lcp1 Kit ELISA, lcp1.S Kit ELISA, Lcp1 Kit ELISA, UL83 Kit ELISA

Sujet:

Gene Name: Lymphocyte Cytosolic Protein 1

Gene Aliases: CP64, LC64P, PLS2, L-Plastin, Plastin 2

ID gène: 3936

UniProt: P13796