CCL20 Kit ELISA

N° du produit ABIN5656588

Détail du produit CCL20 Kit ELISA

Antigène: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.81 pg/mL - 500 pg/mL

Seuil minimal de détection: 7.81 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 3 Alpha (MIP3a). No significant cross-reactivity or interference between Macrophage Inflammatory Protein 3 Alpha (MIP3a) and analogues was observed.

Sensibilité: 2.7 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 3 Alpha (MIP3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 3 Alpha (MIP3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Autre désignation: Macrophage Inflammatory Protein 3 Alpha (CCL20 Produits)

Synonymes: MIP-3ALPHA Kit ELISA, CCL20 Kit ELISA, si:dkey-150o13.1 Kit ELISA, CKb4 Kit ELISA, LARC Kit ELISA, MIP-3-alpha Kit ELISA, MIP-3a Kit ELISA, MIP3A Kit ELISA, SCYA20 Kit ELISA, ST38 Kit ELISA, MIP-3A Kit ELISA, MIP-3[a] Kit ELISA, Scya20 Kit ELISA, exodus-1 Kit ELISA, C-C motif chemokine ligand 20 Kit ELISA, chemokine (C-C motif) ligand 20b Kit ELISA, chemokine (C-C motif) ligand 20 Kit ELISA, CCL20 Kit ELISA, ccl20b Kit ELISA, Ccl20 Kit ELISA

Sujet:

Gene Name: Macrophage Inflammatory Protein 3 Alpha

Gene Aliases: CCL20, CKb4, LARC, MIP3-A, SCYA20, ST38, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20, Beta-Chemokine Exodus-1, Liver And Activation-Regulated Chemokine

ID gène: 6364

UniProt: P78556

Pathways: Phosphorylation & l'infection par le SRAS-CoV-2