M6PR Kit ELISA

N° du produit ABIN5656652

Détail du produit M6PR Kit ELISA

Antigène: M6PR (Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Mannose-6-Phosphate Receptor (M6PR). No significant cross-reactivity or interference between Mannose-6-Phosphate Receptor (M6PR) and analogues was observed.

Sensibilité: 0.63 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose-6-Phosphate Receptor (M6PR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose-6-Phosphate Receptor (M6PR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose-6-Phosphate Receptor (M6PR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose-6-Phosphate Receptor (M6PR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose-6-Phosphate Receptor (M6PR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose-6-Phosphate Receptor (M6PR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: M6PR (Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR))

Autre désignation: Mannose-6-Phosphate Receptor (M6PR Produits)

Synonymes: CD222 Kit ELISA, CIMPR Kit ELISA, M6P-R Kit ELISA, MPR1 Kit ELISA, MPRI Kit ELISA, CD-MPR Kit ELISA, MPR 46 Kit ELISA, MPR-46 Kit ELISA, MPR46 Kit ELISA, SMPR Kit ELISA, Mpr46 Kit ELISA, CDMPR Kit ELISA, m6pr Kit ELISA, MGC68896 Kit ELISA, MGC130765 Kit ELISA, zgc:77757 Kit ELISA, wu:fj81h11 Kit ELISA, MGC89423 Kit ELISA, M6PR Kit ELISA, mprd Kit ELISA, C16orf27 Kit ELISA, GNPTAG Kit ELISA, LP2537 Kit ELISA, RJD9 Kit ELISA, insulin like growth factor 2 receptor Kit ELISA, mannose-6-phosphate receptor, cation dependent Kit ELISA, mannose-6-phosphate receptor (cation dependent) L homeolog Kit ELISA, mannose-6-phosphate receptor (cation dependent) Kit ELISA, mannose-6-phosphate receptor (cation dependent) S homeolog Kit ELISA, Cation-dependent mannose-6-phosphate receptor Kit ELISA, N-acetylglucosamine-1-phosphate transferase gamma subunit Kit ELISA, IGF2R Kit ELISA, M6PR Kit ELISA, M6pr Kit ELISA, m6pr.L Kit ELISA, m6pr Kit ELISA, m6pr.S Kit ELISA, Ethha_1678 Kit ELISA, mprd Kit ELISA, GNPTG Kit ELISA

Sujet:

Gene Name: Mannose-6-Phosphate Receptor

Gene Aliases: SMPR, MPR46, MPRD, Cation Dependent Mannose 6-Phosphate Receptor, 46 kDa mannose 6-phosphate receptor

ID gène: 4074

UniProt: P20645