MARCKSL1 Kit ELISA

N° du produit ABIN5656658

Détail du produit MARCKSL1 Kit ELISA

Antigène: MARCKSL1 (MARCKS-Like 1 (MARCKSL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of MARCKS Related Protein (MARCKSL1). No significant cross-reactivity or interference between MARCKS Related Protein (MARCKSL1) and analogues was observed.

Sensibilité: 0.105 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MARCKS Related Protein (MARCKSL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MARCKS Related Protein (MARCKSL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MARCKS Related Protein (MARCKSL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MARCKS Related Protein (MARCKSL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MARCKS Related Protein (MARCKSL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MARCKS Related Protein (MARCKSL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MARCKSL1 (MARCKS-Like 1 (MARCKSL1))

Autre désignation: MARCKS Related Protein (MARCKSL1 Produits)

Synonymes: F52 Kit ELISA, MACMARCKS Kit ELISA, MLP Kit ELISA, MLP1 Kit ELISA, MRP Kit ELISA, AL022768 Kit ELISA, AW215397 Kit ELISA, AW536807 Kit ELISA, D4Bc1 Kit ELISA, MacMARCKS Kit ELISA, Macs2 Kit ELISA, Macs3 Kit ELISA, Mlp Kit ELISA, Mrp Kit ELISA, cb575 Kit ELISA, marcks1 Kit ELISA, marcksl1 Kit ELISA, sb:cb575 Kit ELISA, wu:fa66h03 Kit ELISA, wu:fj18a04 Kit ELISA, zgc:65840 Kit ELISA, MARCKSL1 Kit ELISA, f52 Kit ELISA, mlp Kit ELISA, mrp Kit ELISA, mlp1 Kit ELISA, macmarcks Kit ELISA, XMLP Kit ELISA, MARCKS like 1 Kit ELISA, MARCKS-like 1 Kit ELISA, MARCKS-like 1a Kit ELISA, MARCKS-like 1 L homeolog Kit ELISA, MARCKS-related protein Kit ELISA, MARCKS-like 1 S homeolog Kit ELISA, MARCKSL1 Kit ELISA, Marcksl1 Kit ELISA, marcksl1a Kit ELISA, marcksl1 Kit ELISA, marcksl1.L Kit ELISA, mrp Kit ELISA, marcksl1.S Kit ELISA

Sujet:

Gene Name: MARCKS Related Protein

Gene Aliases: MLP1, MRP, MLP, F52, MACMARCKS, MARCKS-like protein 1, Macrophage myristoylated alanine-rich C kinase substrate