Melanoma gp100 Kit ELISA

N° du produit ABIN5656810

Détail du produit Melanoma gp100 Kit ELISA

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Melanoma Associated ME20 (ME20M). No significant cross-reactivity or interference between Melanoma Associated ME20 (ME20M) and analogues was observed.

Sensibilité: 0.053 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Associated ME20 (ME20M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Associated ME20 (ME20M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Associated ME20 (ME20M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanoma Associated ME20 (ME20M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanoma Associated ME20 (ME20M) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanoma Associated ME20 (ME20M) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Autre désignation: Melanoma Associated ME20 (PMEL Produits)

Synonymes: D12S53E Kit ELISA, ME20 Kit ELISA, ME20-M Kit ELISA, ME20M Kit ELISA, P1 Kit ELISA, P100 Kit ELISA, PMEL17 Kit ELISA, SI Kit ELISA, SIL Kit ELISA, SILV Kit ELISA, gp100 Kit ELISA, D10H12S53E Kit ELISA, D12S53Eh Kit ELISA, Pmel17 Kit ELISA, Si Kit ELISA, Silv Kit ELISA, gp87 Kit ELISA, RPE1 Kit ELISA, MMP115 Kit ELISA, silverb Kit ELISA, silver Kit ELISA, cb397 Kit ELISA, fdv Kit ELISA, pmel17 Kit ELISA, sb:cb397 Kit ELISA, silva Kit ELISA, wu:fc11g11 Kit ELISA, wu:fj24g11 Kit ELISA, zgc:136622 Kit ELISA, premelanosome protein Kit ELISA, premelanosome protein b Kit ELISA, premelanosome protein a Kit ELISA, PMEL Kit ELISA, Pmel Kit ELISA, pmelb Kit ELISA, pmela Kit ELISA

Sujet:

Gene Name: Melanoma Associated ME20

Gene Aliases: PMEL, GP100, SI, SIL, SILV, Pmel17, Silver Homolog, Premelanosome Protein, Silver Locus Protein Homolog, 95 kDa melanocyte-specific secreted glycoprotein

ID gène: 6490

UniProt: P40967