MANF Kit ELISA

N° du produit ABIN5656834

Détail du produit MANF Kit ELISA

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF). No significant cross-reactivity or interference between Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) and analogues was observed.

Sensibilité: 6.2 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Autre désignation: Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF Produits)

Synonymes: ARMET Kit ELISA, ARP Kit ELISA, 3230402M22Rik Kit ELISA, AA407711 Kit ELISA, AA408789 Kit ELISA, AA673178 Kit ELISA, Armet Kit ELISA, D17914 Kit ELISA, D18Mgi17 Kit ELISA, MANF Kit ELISA, RBM15B Kit ELISA, ARP-like Kit ELISA, CG7013 Kit ELISA, DmMANF Kit ELISA, Dmel\\CG7013 Kit ELISA, anon-EST:ParkEST188 Kit ELISA, arp Kit ELISA, armet Kit ELISA, im:6909481 Kit ELISA, zgc:153327 Kit ELISA, manf Kit ELISA, mesencephalic astrocyte derived neurotrophic factor Kit ELISA, mesencephalic astrocyte-derived neurotrophic factor Kit ELISA, RNA binding motif protein 15B Kit ELISA, Mesencephalic astrocyte-derived neurotrophic factor Kit ELISA, mesencephalic astrocyte-derived neurotrophic factor S homeolog Kit ELISA, MANF Kit ELISA, Manf Kit ELISA, RBM15B Kit ELISA, manf Kit ELISA, manf.S Kit ELISA

Sujet:

Gene Name: Mesencephalic Astrocyte Derived Neurotrophic Factor

Gene Aliases: ARMET, ARP, Arginine Rich Mutated In Early Stage Tumors, Arginine-rich protein