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MTHFR Kit ELISA

MTHFR Reactivité: Humain Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5656870
  • Antigène Voir toutes MTHFR Kits ELISA
    MTHFR (Methylenetetrahydrofolate Reductase (NAD(P)H) (MTHFR))
    Reactivité
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5 pg/mL - 4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Methylenetetrahydrofolate Reductase (MTHFR). No significant cross-reactivity or interference between Methylenetetrahydrofolate Reductase (MTHFR) and analogues was observed.
    Sensibilité
    27.9 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methylenetetrahydrofolate Reductase (MTHFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methylenetetrahydrofolate Reductase (MTHFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methylenetetrahydrofolate Reductase (MTHFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methylenetetrahydrofolate Reductase (MTHFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methylenetetrahydrofolate Reductase (MTHFR) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methylenetetrahydrofolate Reductase (MTHFR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes MTHFR Kits ELISA
    MTHFR (Methylenetetrahydrofolate Reductase (NAD(P)H) (MTHFR))
    Autre désignation
    Methylenetetrahydrofolate Reductase (MTHFR Produits)
    Synonymes
    DDBDRAFT_0204686 Kit ELISA, DDBDRAFT_0230137 Kit ELISA, DDB_0204686 Kit ELISA, DDB_0230137 Kit ELISA, AI323986 Kit ELISA, methylenetetrahydrofolate reductase (NAD(P)H) L homeolog Kit ELISA, methylenetetrahydrofolate reductase Kit ELISA, methylenetetrahydrofolate reductase (NAD(P)H) Kit ELISA, 5,10-methylenetetrahydrofolate reductase Kit ELISA, mthfr.L Kit ELISA, MTHFR Kit ELISA, mthfr Kit ELISA, metF Kit ELISA, DDB_G0279137 Kit ELISA, Mthfr Kit ELISA
    Sujet

    Gene Name: Methylenetetrahydrofolate Reductase

    Gene Aliases: 5,10-Methylenetetrahydrofolate Reductase(NADPH)

    ID gène
    4524
    UniProt
    P42898
    Pathways
    Methionine Biosynthetic Process
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