CD9 Kit ELISA

N° du produit ABIN5656977

Détail du produit CD9 Kit ELISA

Antigène: CD9

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Motility Related Protein (MRP1). No significant cross-reactivity or interference between Motility Related Protein (MRP1) and analogues was observed.

Sensibilité: 12.5 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Motility Related Protein (MRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Motility Related Protein (MRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Motility Related Protein (MRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Motility Related Protein (MRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Motility Related Protein (MRP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Motility Related Protein (MRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CD9

Autre désignation: Motility Related Protein (CD9 Produits)

Synonymes: BTCC-1 Kit ELISA, DRAP-27 Kit ELISA, MIC3 Kit ELISA, MRP-1 Kit ELISA, TSPAN-29 Kit ELISA, TSPAN29 Kit ELISA, Tspan29 Kit ELISA, cd9 Kit ELISA, wu:fb61g04 Kit ELISA, zgc:73383 Kit ELISA, zgc:85743 Kit ELISA, CD9 molecule Kit ELISA, CD9 antigen Kit ELISA, CD9 molecule a Kit ELISA, CD9 Kit ELISA, Cd9 Kit ELISA, cd9a Kit ELISA

Sujet:

Gene Name: Motility Related Protein

Gene Aliases: CD9, TSPAN29, 5H9, BA2, BTCC-1, DRAP-27, GIG2, MIC3, P24, Tetraspanin 29, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3

ID gène: 928

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization