MBP Kit ELISA

N° du produit ABIN5657030

Kit ELISA MBP Humain, Colorimetric test pour la quantification de Humain MBP.

Aperçu rapide pour MBP Kit ELISA (ABIN5657030)

Antigène: MBP (Myelin Basic Protein (MBP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 3.12 ng/mL - 200 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit MBP Kit ELISA

Seuil minimal de détection: 3.12 ng/mL

Fonction: Human Myelin Basic Protein (MBP) Anti-antigen ELISA Kit for detecting MBP in serum, plasma

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Myelin Basic Protein (MBP). No significant cross-reactivity or interference between Myelin Basic Protein (MBP) and analogues was observed.

Sensibilité: 1.18 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 100 μL

Durée du test: 2.5 h

Plaque: Pre-coated

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myelin Basic Protein (MBP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myelin Basic Protein (MBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: MBP (Myelin Basic Protein (MBP))

Autre désignation: Myelin Basic Protein

Sujet: Gene Aliases: Myelin A1 protein, Myelin membrane encephalitogenic protein

ID gène: 4155

UniProt: P02686