MYL3/CMLC1 Kit ELISA

N° du produit ABIN5657105

Détail du produit MYL3/CMLC1 Kit ELISA

Antigène: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.12 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3). No significant cross-reactivity or interference between Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) and analogues was observed.

Sensibilité: 30 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Autre désignation: Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3 Produits)

Synonymes: CMD1S Kit ELISA, CMH1 Kit ELISA, MPD1 Kit ELISA, MYHCB Kit ELISA, SPMD Kit ELISA, SPMM Kit ELISA, B-MHC Kit ELISA, MyHC-I Kit ELISA, Myhc-b Kit ELISA, Myhcb Kit ELISA, beta-MHC Kit ELISA, MLC1s Kit ELISA, MLC1v Kit ELISA, Mylc Kit ELISA, VLC1 Kit ELISA, Mylc1v Kit ELISA, CMH8 Kit ELISA, MLC1SB Kit ELISA, MLC1V Kit ELISA, mlc1v Kit ELISA, myl3-a Kit ELISA, myl3-b Kit ELISA, myl3.L Kit ELISA, zgc:103441 Kit ELISA, myosin heavy chain 7 Kit ELISA, myosin, heavy polypeptide 7, cardiac muscle, beta Kit ELISA, myosin, light polypeptide 3 Kit ELISA, myosin light chain 3 Kit ELISA, myosin light chain 3 S homeolog Kit ELISA, cardiac myosin light chain-1 Kit ELISA, MYH7 Kit ELISA, Myh7 Kit ELISA, Myl3 Kit ELISA, MYL3 Kit ELISA, myl3.S Kit ELISA, cmlc1 Kit ELISA

Sujet:

Gene Name: Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal

Gene Aliases: CMH8, MLC1V, VLC1, MLC1SB, Cardiac myosin light chain 1, Ventricular/slow twitch myosin alkali light chain

ID gène: 4634

UniProt: P08590