ASAH1 Kit ELISA

N° du produit ABIN5657146

Détail du produit ASAH1 Kit ELISA

Antigène: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of N-Acylsphingosine Amidohydrolase 1 (ASAH1). No significant cross-reactivity or interference between N-Acylsphingosine Amidohydrolase 1 (ASAH1) and analogues was observed.

Sensibilité: 0.63 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acylsphingosine Amidohydrolase 1 (ASAH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Acylsphingosine Amidohydrolase 1 (ASAH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acylsphingosine Amidohydrolase 1 (ASAH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Acylsphingosine Amidohydrolase 1 (ASAH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-Acylsphingosine Amidohydrolase 1 (ASAH1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-Acylsphingosine Amidohydrolase 1 (ASAH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Autre désignation: N-Acylsphingosine Amidohydrolase 1 (ASAH1 Produits)

Synonymes: AC Kit ELISA, ACDase Kit ELISA, ASAH Kit ELISA, PHP Kit ELISA, PHP32 Kit ELISA, SMAPME Kit ELISA, 2310081N20Rik Kit ELISA, Asah Kit ELISA, MGC82286 Kit ELISA, asah1 Kit ELISA, zgc:101637 Kit ELISA, ASAH1 Kit ELISA, zgc:66026 Kit ELISA, N-acylsphingosine amidohydrolase 1 Kit ELISA, N-acylsphingosine amidohydrolase (acid ceramidase) 1 Kit ELISA, N-acylsphingosine amidohydrolase (acid ceramidase) 1 L homeolog Kit ELISA, N-acylsphingosine amidohydrolase (acid ceramidase) 1a Kit ELISA, Acid ceramidase Kit ELISA, N-acylsphingosine amidohydrolase (acid ceramidase) 1b Kit ELISA, ASAH1 Kit ELISA, Asah1 Kit ELISA, asah1.L Kit ELISA, asah1a Kit ELISA, asah-1 Kit ELISA, asah1b Kit ELISA

Sujet:

Gene Name: N-Acylsphingosine Amidohydrolase 1

Gene Aliases: AC, ASAH, PHP, PHP32, Acid Ceramidase, Acylsphingosine deacylase, Putative 32 kDa heart protein

ID gène: 427

UniProt: Q13510