Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

MT-ND5 Kit ELISA

MT-ND5 Reactivité: Humain Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5657175
  • Antigène Voir toutes MT-ND5 Kits ELISA
    MT-ND5 (Mitochondrially Encoded NADH Dehydrogenase 5 (MT-ND5))
    Reactivité
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5 pg/mL - 4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of NADH Dehydrogenase 5 (ND5). No significant cross-reactivity or interference between NADH Dehydrogenase 5 (ND5) and analogues was observed.
    Sensibilité
    27.4 pg/mL
    Featured
    Discover our best selling MT-ND5 Kit ELISA
    Top Product
    Discover our top product MT-ND5 Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH Dehydrogenase 5 (ND5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADH Dehydrogenase 5 (ND5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADH Dehydrogenase 5 (ND5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NADH Dehydrogenase 5 (ND5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NADH Dehydrogenase 5 (ND5) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NADH Dehydrogenase 5 (ND5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes MT-ND5 Kits ELISA
    MT-ND5 (Mitochondrially Encoded NADH Dehydrogenase 5 (MT-ND5))
    Autre désignation
    NADH Dehydrogenase 5 (MT-ND5 Produits)
    Synonymes
    MTND5 Kit ELISA, NADH5 Kit ELISA, NADH dehydrogenase, subunit 5 (complex I) Kit ELISA, NADH dehydrogenase subunit 5 Kit ELISA, NADH dehydrogenasesubunit 5 Kit ELISA, mitochondrial NADH-ubiquinone oxidoreductase chain 5 Kit ELISA, ND5 Kit ELISA, nad5 Kit ELISA, mt:ND5 Kit ELISA
    Sujet

    Gene Name: NADH Dehydrogenase 5

    Gene Aliases: MT-ND5, MTND5, NAD5, NADH-Ubiquinone Oxidoreductase Chain 5, Mitochondrially Encoded NADH Dehydrogenase 5

    ID gène
    4540
    UniProt
    P03915
Vous êtes ici:
Support technique