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Neuropilin 1 Kit ELISA

NRP1 Reactivité: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5657329
  • Antigène Voir toutes Neuropilin 1 (NRP1) Kits ELISA
    Neuropilin 1 (NRP1)
    Reactivité
    • 6
    • 5
    • 3
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Neuropilin 1 (NRP1). No significant cross-reactivity or interference between Neuropilin 1 (NRP1) and analogues was observed.
    Sensibilité
    0.053 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropilin 1 (NRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuropilin 1 (NRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuropilin 1 (NRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuropilin 1 (NRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropilin 1 (NRP1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropilin 1 (NRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Neuropilin 1 (NRP1) Kits ELISA
    Neuropilin 1 (NRP1)
    Autre désignation
    Neuropilin 1 (NRP1 Produits)
    Synonymes
    NRP1 Kit ELISA, cd304 Kit ELISA, neuropilin Kit ELISA, np-1 Kit ELISA, npn-1 Kit ELISA, nrp Kit ELISA, nrp-1 Kit ELISA, vegf165r Kit ELISA, BDCA4 Kit ELISA, CD304 Kit ELISA, NP1 Kit ELISA, NRP Kit ELISA, VEGF165R Kit ELISA, C530029I03 Kit ELISA, NP-1 Kit ELISA, NPN-1 Kit ELISA, Npn1 Kit ELISA, Nrp Kit ELISA, neuropilin 1 Kit ELISA, neuropilin 1 L homeolog Kit ELISA, NRP1 Kit ELISA, nrp1 Kit ELISA, Nrp1 Kit ELISA, nrp1.L Kit ELISA
    Sujet

    Gene Name: Neuropilin 1

    Gene Aliases: CD304, BDCA4, VEGF165R, Vascular endothelial cell growth factor 165 receptor

    ID gène
    246331
    UniProt
    Q9QWJ9
    Pathways
    Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals
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