Lipocalin 2 Kit CLIA
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- Antigène Voir toutes Lipocalin 2 (LCN2) Kits CLIA
- Lipocalin 2 (LCN2)
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Reactivité
- Humain
- Méthode de détection
- Chemiluminescent
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 78.12 pg/mL - 20000 pg/mL
- Seuil minimal de détection
- 78.12 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Neutrophil Gelatinase Associated Lipocalin (NGAL). No significant cross-reactivity or interference between Neutrophil Gelatinase Associated Lipocalin (NGAL) and analogues was observed.
- Sensibilité
- 9.8 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 2 - 3 h
- Plaque
- Pre-coated
- Protocole
- The microplate provided in this kit has been pre-coated with an antibody specific to Neutrophil Gelatinase Associated Lipocalin (NGAL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Neutrophil Gelatinase Associated Lipocalin (NGAL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Neutrophil Gelatinase Associated Lipocalin (NGAL) level in the sample or standard.,
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil Gelatinase Associated Lipocalin (NGAL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil Gelatinase Associated Lipocalin (NGAL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes Lipocalin 2 (LCN2) Kits CLIA
- Lipocalin 2 (LCN2)
- Autre désignation
- Neutrophil Gelatinase Associated Lipocalin (LCN2 Produits)
- Synonymes
- LCN2 Kit CLIA, 24p3 Kit CLIA, MSFI Kit CLIA, NGAL Kit CLIA, AW212229 Kit CLIA, Sip24 Kit CLIA, lipocalin 2 Kit CLIA, LCN2 Kit CLIA, Lcn2 Kit CLIA
- Sujet
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Gene Name: Neutrophil Gelatinase Associated Lipocalin
Gene Aliases: LCN2, p25, Lipocalin 2, Oncogene 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Siderocalin LCN2
- ID gène
- 3934
- UniProt
- P80188
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis
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