NOS2 Kit ELISA

N° du produit ABIN5657403

Détail du produit NOS2 Kit ELISA

Antigène: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Nitric Oxide Synthase 2, Inducible (NOS2). No significant cross-reactivity or interference between Nitric Oxide Synthase 2, Inducible (NOS2) and analogues was observed.

Sensibilité: 0.116 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase 2, Inducible (NOS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase 2, Inducible (NOS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase 2, Inducible (NOS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Synthase 2, Inducible (NOS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitric Oxide Synthase 2, Inducible (NOS2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitric Oxide Synthase 2, Inducible (NOS2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Autre désignation: Nitric Oxide Synthase 2, Inducible (NOS2 Produits)

Synonymes: HEP-NOS Kit ELISA, INOS Kit ELISA, NOS Kit ELISA, NOS2A Kit ELISA, NOS-II Kit ELISA, Nos-2 Kit ELISA, Nos2a Kit ELISA, i-NOS Kit ELISA, iNOS Kit ELISA, iNos Kit ELISA, NOS2a Kit ELISA, NOS2 Kit ELISA, BmNOS2 Kit ELISA, Nsl Kit ELISA, nos2 Kit ELISA, nitric oxide synthase 2 Kit ELISA, nitric oxide synthase 2, inducible Kit ELISA, inducible nitric oxide synthase Kit ELISA, inducible nitric-oxide synthase Kit ELISA, nanos C2HC-type zinc finger 2 Kit ELISA, NOS2 Kit ELISA, Nos2 Kit ELISA, nos2 Kit ELISA, LOC396821 Kit ELISA, NANOS2 Kit ELISA

Sujet:

Gene Name: Nitric Oxide Synthase 2, Inducible

Gene Aliases: NOS2A, INOS, HEP-NOS, I-NOS, Hepatocytes Oxide Synthase, Peptidyl-cysteine S-nitrosylase NOS2

ID gène: 18126

UniProt: P29477

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process