OCT4 Kit ELISA
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- Antigène Voir toutes OCT4 (POU5F1) Kits ELISA
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Octamer Binding Transcription Factor 4 (OCT4). No significant cross-reactivity or interference between Octamer Binding Transcription Factor 4 (OCT4) and analogues was observed.
- Sensibilité
- 0.061 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Octamer Binding Transcription Factor 4 (OCT4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Octamer Binding Transcription Factor 4 (OCT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Octamer Binding Transcription Factor 4 (OCT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Octamer Binding Transcription Factor 4 (OCT4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Octamer Binding Transcription Factor 4 (OCT4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Octamer Binding Transcription Factor 4 (OCT4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes OCT4 (POU5F1) Kits ELISA
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
- Autre désignation
- Octamer Binding Transcription Factor 4 (POU5F1 Produits)
- Synonymes
- OCT3 Kit ELISA, OCT4 Kit ELISA, OTF-3 Kit ELISA, OTF3 Kit ELISA, OTF4 Kit ELISA, Oct-3 Kit ELISA, Oct-4 Kit ELISA, POU5F1 Kit ELISA, gp9 Kit ELISA, gp-9 Kit ELISA, oct4 Kit ELISA, pou2 Kit ELISA, POU-2 Kit ELISA, cb197 Kit ELISA, spg/pou2 Kit ELISA, chunp6868 Kit ELISA, wu:fd18d06 Kit ELISA, NF-A3 Kit ELISA, Oct-3/4 Kit ELISA, Oct3 Kit ELISA, Oct3/4 Kit ELISA, Oct4 Kit ELISA, Otf-3 Kit ELISA, Otf-4 Kit ELISA, Otf3 Kit ELISA, Otf3-rs7 Kit ELISA, Otf3g Kit ELISA, Otf4 Kit ELISA, POU class 5 homeobox 1 Kit ELISA, POU domain, class 5, transcription factor 3 Kit ELISA, POU domain, class 5, transcription factor 1 Kit ELISA, POU5F1 Kit ELISA, pou5f3 Kit ELISA, Pou5f1 Kit ELISA
- Sujet
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Gene Name: Octamer Binding Transcription Factor 4
Gene Aliases: POU5F1, OCT3, OTF3, OTF4, Octamer-4, POU Domain Class 5 Transcription Factor 1, POU Class 5 Homoeobox 1, Octamer-binding protein 3
- ID gène
- 5460
- UniProt
- Q01860
- Pathways
- Stem Cell Maintenance
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