OTC Kit ELISA

N° du produit ABIN5657520

Détail du produit OTC Kit ELISA

Antigène: OTC (Ornithine Carbamoyltransferase (OTC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ornithine Carbamoyl Transferase (OCT). No significant cross-reactivity or interference between Ornithine Carbamoyl Transferase (OCT) and analogues was observed.

Sensibilité: 0.128 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Carbamoyl Transferase (OCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ornithine Carbamoyl Transferase (OCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ornithine Carbamoyl Transferase (OCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ornithine Carbamoyl Transferase (OCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ornithine Carbamoyl Transferase (OCT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ornithine Carbamoyl Transferase (OCT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: OTC (Ornithine Carbamoyltransferase (OTC))

Autre désignation: Ornithine Carbamoyl Transferase (OTC Produits)

Synonymes: OCTD Kit ELISA, 2810428A13Rik Kit ELISA, AA589422 Kit ELISA, AW457381 Kit ELISA, OCT Kit ELISA, Plxn2 Kit ELISA, mKIAA0463 Kit ELISA, F1B16.13 Kit ELISA, F1B16_13 Kit ELISA, ORNITHINE CARBAMOYLTRANSFERASE Kit ELISA, ornithine carbamoyltransferase Kit ELISA, BA4351 Kit ELISA, PSPTO4164 Kit ELISA, PLXN2 Kit ELISA, AI265390 Kit ELISA, Sf Kit ELISA, spf Kit ELISA, si:dkey-19h21.3 Kit ELISA, ornithine carbamoyltransferase Kit ELISA, plexin A2 Kit ELISA, ornithine carbamoyltransferase ArgF Kit ELISA, ornithine transcarbamylase Kit ELISA, OTC Kit ELISA, Plxna2 Kit ELISA, Otc Kit ELISA, argF Kit ELISA, argF-2 Kit ELISA, atpD-2 Kit ELISA, CNC04300 Kit ELISA, PLXNA2 Kit ELISA, otc Kit ELISA

Sujet:

Gene Name: Ornithine Carbamoyl Transferase

Gene Aliases: OCTD, OTCase, Ornithine Transcarbamylase, Ornithine Carbamoyltransferase

ID gène: 5009

UniProt: P00480