ODC1 Kit ELISA

N° du produit ABIN5657525

Détail du produit ODC1 Kit ELISA

Antigène: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ornithine Decarboxylase (ODC). No significant cross-reactivity or interference between Ornithine Decarboxylase (ODC) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Decarboxylase (ODC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ornithine Decarboxylase (ODC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ornithine Decarboxylase (ODC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ornithine Decarboxylase (ODC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ornithine Decarboxylase (ODC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ornithine Decarboxylase (ODC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Autre désignation: Ornithine Decarboxylase (ODC1 Produits)

Synonymes: CG8721 Kit ELISA, Dmel\\CG8721 Kit ELISA, dodc1 Kit ELISA, odc Kit ELISA, fc54f04 Kit ELISA, fi06d08 Kit ELISA, CHUNP6922 Kit ELISA, wu:fc54f04 Kit ELISA, wu:fi06d08 Kit ELISA, odc2 Kit ELISA, xODC1 Kit ELISA, xodc2 Kit ELISA, DDBDRAFT_0204020 Kit ELISA, DDBDRAFT_0237755 Kit ELISA, DDB_0204020 Kit ELISA, DDB_0237755 Kit ELISA, ODC Kit ELISA, odc1 Kit ELISA, ODC1 Kit ELISA, Odc Kit ELISA, odc1-a Kit ELISA, Ornithine decarboxylase 1 Kit ELISA, ornithine decarboxylase 1 Kit ELISA, ornithine decarboxylase Kit ELISA, diaminopimelate decarboxylase Kit ELISA, Orn/DAP/Arg decarboxylase 2 domain-containing protein Kit ELISA, Diaminopimelate decarboxylase Kit ELISA, Ornithine decarboxylase Kit ELISA, ornithine decarboxylase 1 L homeolog Kit ELISA, ornithine decarboxylase, structural 1 Kit ELISA, Odc1 Kit ELISA, odc1 Kit ELISA, ODC1 Kit ELISA, odc Kit ELISA, RR_RS04895 Kit ELISA, ASA_RS06380 Kit ELISA, LOC100232922 Kit ELISA, odc1.L Kit ELISA, odc-1 Kit ELISA

Sujet:

Gene Name: Ornithine Decarboxylase

Gene Aliases: ODC1

ID gène: 24609

UniProt: P09057