Osteopontin Kit ELISA
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- Antigène Voir toutes Osteopontin (SPP1) Kits ELISA
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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Reactivité
- Chien
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 3.12 pg/mL - 200 pg/mL
- Seuil minimal de détection
- 3.12 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Osteopontin (OPN). No significant cross-reactivity or interference between Osteopontin (OPN) and analogues was observed.
- Sensibilité
- 1.02 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteopontin (OPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteopontin (OPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteopontin (OPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osteopontin (OPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteopontin (OPN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteopontin (OPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes Osteopontin (SPP1) Kits ELISA
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- Autre désignation
- Osteopontin (SPP1 Produits)
- Synonymes
- BNSP Kit ELISA, BSPI Kit ELISA, ETA-1 Kit ELISA, OPN Kit ELISA, 2AR Kit ELISA, Apl-1 Kit ELISA, Bsp Kit ELISA, Eta Kit ELISA, OP Kit ELISA, Opn Kit ELISA, Opnl Kit ELISA, Ric Kit ELISA, Spp-1 Kit ELISA, OSP Kit ELISA, zgc:111821 Kit ELISA, secreted phosphoprotein 1 Kit ELISA, SPP1 Kit ELISA, Spp1 Kit ELISA, spp1 Kit ELISA
- Sujet
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Gene Name: Osteopontin
Gene Aliases: SPP1, BNSP, BSPI, ETA1, Secreted Phosphoprotein 1, Bone Sialoprotein I, Early T-Lymphocyte Activation 1, Nephropontin, Urinary stone protein, Uropontin
- UniProt
- Q3HTT3
- Pathways
- Regulation of Cell Size
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