PAM Kit ELISA

N° du produit ABIN5657690

Détail du produit PAM Kit ELISA

Antigène: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cerebrospinal Fluid, Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Peptidylglycine Alpha Amidating Monooxygenase (PAM). No significant cross-reactivity or interference between Peptidylglycine Alpha Amidating Monooxygenase (PAM) and analogues was observed.

Sensibilité: 13.5 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidylglycine Alpha Amidating Monooxygenase (PAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidylglycine Alpha Amidating Monooxygenase (PAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidylglycine Alpha Amidating Monooxygenase (PAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidylglycine Alpha Amidating Monooxygenase (PAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidylglycine Alpha Amidating Monooxygenase (PAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidylglycine Alpha Amidating Monooxygenase (PAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Autre désignation: Peptidylglycine Alpha Amidating Monooxygenase (PAM Produits)

Synonymes: PAL Kit ELISA, PHM Kit ELISA, pal Kit ELISA, phm Kit ELISA, pamb Kit ELISA, CG3832 Kit ELISA, Dmel\\CG3832 Kit ELISA, dPHM Kit ELISA, AE-I Kit ELISA, PAL-A Kit ELISA, PHM-A Kit ELISA, pama Kit ELISA, si:dkeyp-35f12.2 Kit ELISA, peptidylglycine alpha-amidating monooxygenase Kit ELISA, peptidylglycine alpha-amidating monooxygenase S homeolog Kit ELISA, Peptidylglycine-alpha-hydroxylating monooxygenase Kit ELISA, peptidylglycine alpha-amidating monooxygenase L homeolog Kit ELISA, PAM Kit ELISA, Pam Kit ELISA, pam.S Kit ELISA, CHLREDRAFT_150435 Kit ELISA, sce7070 Kit ELISA, pam Kit ELISA, Phm Kit ELISA, pam.L Kit ELISA

Sujet:

Gene Name: Peptidylglycine Alpha Amidating Monooxygenase

Gene Aliases: PAL, PHM, Peptidylamidoglycolate lyase, Peptidyl-Alpha-hydroxyglycine Alpha-amidating Lyase, Peptidylglycine Alpha-Hydroxylating Monooxygenase

ID gène: 5066

UniProt: P19021