PEX2 Kit ELISA

N° du produit ABIN5657731

Détail du produit PEX2 Kit ELISA

Antigène: PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Peroxisomal Biogenesis Factor 2 (PEX2). No significant cross-reactivity or interference between Peroxisomal Biogenesis Factor 2 (PEX2) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisomal Biogenesis Factor 2 (PEX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisomal Biogenesis Factor 2 (PEX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisomal Biogenesis Factor 2 (PEX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisomal Biogenesis Factor 2 (PEX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisomal Biogenesis Factor 2 (PEX2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisomal Biogenesis Factor 2 (PEX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))

Autre désignation: Peroxisomal Biogenesis Factor 2 (PEX2 Produits)

Synonymes: PAF1 Kit ELISA, PBD5A Kit ELISA, PBD5B Kit ELISA, PMP3 Kit ELISA, PMP35 Kit ELISA, PXMP3 Kit ELISA, RNF72 Kit ELISA, ZWS3 Kit ELISA, D3Ertd138e Kit ELISA, PAF-1 Kit ELISA, Pxmp3 Kit ELISA, pxmp3 Kit ELISA, An18g04150 Kit ELISA, DDBDRAFT_0168812 Kit ELISA, DDBDRAFT_0238045 Kit ELISA, DDB_0168812 Kit ELISA, DDB_0238045 Kit ELISA, peroxisomal biogenesis factor 2 Kit ELISA, RING zinc finger-containing protein Kit ELISA, PEX2 Kit ELISA, Pex2 Kit ELISA, pex2 Kit ELISA, ANI_1_1336164 Kit ELISA, HCAG_04580 Kit ELISA, CC1G_00418 Kit ELISA, PTRG_10616 Kit ELISA, PAAG_05772 Kit ELISA, MGYG_06097 Kit ELISA

Sujet:

Gene Name: Peroxisomal Biogenesis Factor 2

Gene Aliases: PXMP3, PAF1, PMP35, PMP3, RNF72, Peroxin 2, Peroxisome Assembly Factor 1, Peroxisomal Membrane Protein 35 kDa, Peroxisomal Membrane Protein 3

ID gène: 5828

UniProt: P28328

Pathways: Monocarboxylic Acid Catabolic Process