PEBP1 Kit ELISA

N° du produit ABIN5657756

Détail du produit PEBP1 Kit ELISA

Antigène: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Phosphatidylethanolamine Binding Protein 1 (PEBP1). No significant cross-reactivity or interference between Phosphatidylethanolamine Binding Protein 1 (PEBP1) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylethanolamine Binding Protein 1 (PEBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphatidylethanolamine Binding Protein 1 (PEBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphatidylethanolamine Binding Protein 1 (PEBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatidylethanolamine Binding Protein 1 (PEBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphatidylethanolamine Binding Protein 1 (PEBP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphatidylethanolamine Binding Protein 1 (PEBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Autre désignation: Phosphatidylethanolamine Binding Protein 1 (PEBP1 Produits)

Synonymes: HCNP Kit ELISA, HCNPpp Kit ELISA, PBP Kit ELISA, PEBP Kit ELISA, PEBP-1 Kit ELISA, RKIP Kit ELISA, pbp Kit ELISA, hcnp Kit ELISA, pebp Kit ELISA, rkip Kit ELISA, Pbp Kit ELISA, Pbp1 Kit ELISA, Pbpr Kit ELISA, Rkip Kit ELISA, PEBP1 Kit ELISA, BcDNA:LP12095 Kit ELISA, CG18594 Kit ELISA, Dmel\\CG18594 Kit ELISA, T2 Kit ELISA, zgc:56033 Kit ELISA, zgc:76942 Kit ELISA, phosphatidylethanolamine binding protein 1 Kit ELISA, phosphatidylethanolamine binding protein 1 L homeolog Kit ELISA, phosphatidylethanolamine-binding protein Kit ELISA, phosphatidylethanolamine-binding protein,putative Kit ELISA, Phosphatidylethanolamine-binding protein 1 Kit ELISA, PEBP1 Kit ELISA, pebp1 Kit ELISA, Pebp1 Kit ELISA, pebp1.L Kit ELISA, RR_RS07785 Kit ELISA, PVX_235290 Kit ELISA, PVX_123630 Kit ELISA, PKH_143640 Kit ELISA, EAM_1207 Kit ELISA

Sujet:

Gene Name: Phosphatidylethanolamine Binding Protein 1

Gene Aliases: PBP, HCNP, PEBP, RKIP, Prostatic Binding Protein, Raf Kinase Inhibitory Protein, Hippocampal Cholinergic Neurostimulating Peptide, Neuropolypeptide h3

ID gène: 5037

UniProt: P30086

Pathways: Feeding Behaviour