PCK1 Kit ELISA

N° du produit ABIN5657771

Détail du produit PCK1 Kit ELISA

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1). No significant cross-reactivity or interference between Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Autre désignation: Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1 Produits)

Synonymes: PEPCK-C Kit ELISA, PEPCK1 Kit ELISA, PEPCKC Kit ELISA, PEPCK Kit ELISA, PEPCK-M Kit ELISA, PEPCK2 Kit ELISA, 1810010O14Rik Kit ELISA, 9130022B02Rik Kit ELISA, cb856 Kit ELISA, cb924 Kit ELISA, fj93h11 Kit ELISA, zgc:63869 Kit ELISA, wu:fc51c05 Kit ELISA, wu:fj93h11 Kit ELISA, pepck-c Kit ELISA, pepck1 Kit ELISA, pepckc Kit ELISA, PCK1 Kit ELISA, PPCK1 Kit ELISA, AI265463 Kit ELISA, Pck-1 Kit ELISA, GTP Kit ELISA, PCK Kit ELISA, Pepck Kit ELISA, RATPEPCK Kit ELISA, Ppc1C Kit ELISA, PHOSPHOENOLPYRUVATE CARBOXYKINASE Kit ELISA, T28I19.150 Kit ELISA, T28I19_150 Kit ELISA, phosphoenolpyruvate carboxykinase 1 Kit ELISA, 143299_at Kit ELISA, CG10924 Kit ELISA, CG17725 Kit ELISA, Dmel\\CG17725 Kit ELISA, Dromel_CG17725_FBtr0086701_pepck_mORF Kit ELISA, dPEPCK Kit ELISA, pepck Kit ELISA, PEPC Kit ELISA, PEPCase Kit ELISA, ppc Kit ELISA, phosphoenolpyruvate carboxykinase 1 Kit ELISA, phosphoenolpyruvate carboxykinase 2, mitochondrial Kit ELISA, phosphoenolpyruvate carboxykinase 2 (mitochondrial) Kit ELISA, phosphoenolpyruvate carboxykinase 1 (soluble) Kit ELISA, phosphoenolpyruvate carboxykinase 1 S homeolog Kit ELISA, phosphoenolpyruvate carboxykinase, cytosolic [GTP] Kit ELISA, phosphoenolpyruvate carboxykinase 1, cytosolic Kit ELISA, phosphoenolpyruvate carboxylase 7 Kit ELISA, Phosphoenolpyruvate carboxykinase Kit ELISA, phosphoenolpyruvate carboxylase Kit ELISA, PCK1 Kit ELISA, PCK2 Kit ELISA, Pck2 Kit ELISA, pck1 Kit ELISA, pck1.S Kit ELISA, LOC100634531 Kit ELISA, Pck1 Kit ELISA, pep7 Kit ELISA, Pepck Kit ELISA, LOC107777405 Kit ELISA

Sujet:

Gene Name: Phosphoenolpyruvate Carboxykinase 1, Soluble

Gene Aliases: PEPCK1, PEPCKC, Cytosolic Phosphoenolpyruvate Carboxykinase, Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

ID gène: 18534

UniProt: Q9Z2V4

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis