PLAA Kit ELISA
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- Antigène Voir toutes PLAA Kits ELISA
- PLAA (Phospholipase A2-Activating Protein (PLAA))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.312 ng/mL - 20 ng/mL
- Seuil minimal de détection
- 0.312 ng/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Phospholipase A2 Activating Protein (PLAA). No significant cross-reactivity or interference between Phospholipase A2 Activating Protein (PLAA) and analogues was observed.
- Sensibilité
- 0.105 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2 Activating Protein (PLAA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2 Activating Protein (PLAA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2 Activating Protein (PLAA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2 Activating Protein (PLAA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2 Activating Protein (PLAA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2 Activating Protein (PLAA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes PLAA Kits ELISA
- PLAA (Phospholipase A2-Activating Protein (PLAA))
- Autre désignation
- Phospholipase A2 Activating Protein (PLAA Produits)
- Synonymes
- cb623 Kit ELISA, zgc:56568 Kit ELISA, zgc:66193 Kit ELISA, wu:fc45d08 Kit ELISA, wu:fi33e06 Kit ELISA, PLAA Kit ELISA, doa1 Kit ELISA, pla2p Kit ELISA, plap Kit ELISA, DOA1 Kit ELISA, PLA2P Kit ELISA, PLAP Kit ELISA, 2410007N06 Kit ELISA, AI536418 Kit ELISA, AU018445 Kit ELISA, AW208417 Kit ELISA, D4Ertd618e Kit ELISA, Ufd3 Kit ELISA, Plap Kit ELISA, phospholipase A2-activating protein Kit ELISA, phospholipase A2 activating protein Kit ELISA, phospholipase A2-activating protein L homeolog Kit ELISA, phospholipase A2-activating protein S homeolog Kit ELISA, phospholipase A2, activating protein Kit ELISA, plaa Kit ELISA, PLAA Kit ELISA, plaa.L Kit ELISA, plaa.S Kit ELISA, Plaa Kit ELISA
- Sujet
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Gene Name: Phospholipase A2 Activating Protein
Gene Aliases: PLAP, PLA2P, PLA2-P, DOA1
- ID gène
- 9373
- UniProt
- Q9Y263
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