PLSCR1 Kit ELISA

N° du produit ABIN5657814

Détail du produit PLSCR1 Kit ELISA

Antigène: PLSCR1 (phospholipid Scramblase 1 (PLSCR1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Phospholipid Scramblase 1 (PLSCR1). No significant cross-reactivity or interference between Phospholipid Scramblase 1 (PLSCR1) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipid Scramblase 1 (PLSCR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipid Scramblase 1 (PLSCR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipid Scramblase 1 (PLSCR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipid Scramblase 1 (PLSCR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipid Scramblase 1 (PLSCR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipid Scramblase 1 (PLSCR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PLSCR1 (phospholipid Scramblase 1 (PLSCR1))

Autre désignation: Phospholipid Scramblase 1 (PLSCR1 Produits)

Synonymes: mmtra1b Kit ELISA, MGC84969 Kit ELISA, PLSCR1 Kit ELISA, MGC148322 Kit ELISA, MMTRA1B Kit ELISA, MmTRA1a Kit ELISA, MmTRA1b Kit ELISA, Nor1 Kit ELISA, Tra1 Kit ELISA, Tra1a Kit ELISA, Tra1b Kit ELISA, Tras1 Kit ELISA, Tras2 Kit ELISA, PLSCR2 Kit ELISA, phospholipid scramblase 1 L homeolog Kit ELISA, phospholipid scramblase 1 Kit ELISA, plscr1.L Kit ELISA, PLS1 Kit ELISA, PVX_111580 Kit ELISA, PLSCR1 Kit ELISA, plscr1 Kit ELISA, Plscr1 Kit ELISA, LOC100712949 Kit ELISA, PLSCR2 Kit ELISA, LOC100341366 Kit ELISA, LOC611500 Kit ELISA

Sujet:

Gene Name: Phospholipid Scramblase 1

Gene Aliases: MMTRA1B, Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase

ID gène: 5359

UniProt: O15162

Pathways: Cellular Response to Molecule of Bacterial Origin