PF4 Kit ELISA

N° du produit ABIN5657929

Détail du produit PF4 Kit ELISA

Antigène: PF4 (Platelet Factor 4 (PF4))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Platelet-Poor Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Platelet Factor 4 (PF4). No significant cross-reactivity or interference between Platelet Factor 4 (PF4) and analogues was observed.

Sensibilité: 0.072 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Factor 4 (PF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Factor 4 (PF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Factor 4 (PF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Factor 4 (PF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Factor 4 (PF4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Factor 4 (PF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PF4 (Platelet Factor 4 (PF4))

Autre désignation: Platelet Factor 4 (PF4 Produits)

Synonymes: CXCL4 Kit ELISA, PF-4 Kit ELISA, SCYB4 Kit ELISA, Cxcl4 Kit ELISA, Scyb4 Kit ELISA, Pf4a Kit ELISA, RATPF4A Kit ELISA, PF4A Kit ELISA, CXCL4L1 Kit ELISA, CXCL4V1 Kit ELISA, PF4-ALT Kit ELISA, SCYB4V1 Kit ELISA, PF4 Kit ELISA, platelet factor 4 Kit ELISA, platelet factor 4 variant 1 Kit ELISA, PF4 Kit ELISA, Pf4 Kit ELISA, LOC703451 Kit ELISA, PF4V1 Kit ELISA, LOC100524373 Kit ELISA, LOC102181854 Kit ELISA, LOC100630489 Kit ELISA

Sujet:

Gene Name: Platelet Factor 4

Gene Aliases: CXCL4, SCYB4, Chemokine C-X-C-Motif Ligand 4, Oncostatin-A, Iroplact

ID gène: 56744

UniProt: Q9Z126