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POTEG Kit CLIA

Ce kit ELISA Humain POTEG est un kit ELISA Chemiluminescent conçu pour quantifier Humain POTEG.
N° du produit ABIN5657992

Aperçu rapide pour POTEG Kit CLIA (ABIN5657992)

Antigène

Tous les produits POTEG
POTEG (POTE Ankyrin Domain Family, Member G (POTEG))

Reactivité

Humain

Méthode de détection

Chemiluminescent

Type de méthode

Sandwich ELISA

Gamme de detection

312.5 pg/mL - 20000 pg/mL

Application

ELISA

Type d'échantillon

Cell Lysate, Tissue Homogenate
  • Seuil minimal de détection

    312.5 pg/mL

    Analytical Method

    Quantitative

    Specificité

    This assay has high sensitivity and excellent specificity for detection of POTE Ankyrin Domain Family, Member G (POTEG). No significant cross-reactivity or interference between POTE Ankyrin Domain Family, Member G (POTEG) and analogues was observed.

    Sensibilité

    11.21 pg/mL
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test

    2 - 3 h

    Plaque

    Pre-coated

    Protocole

    The microplate provided in this kit has been pre-coated with an antibody specific to POTE Ankyrin Domain Family, Member G (POTEG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to POTE Ankyrin Domain Family, Member G (POTEG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the POTE Ankyrin Domain Family, Member G (POTEG) level in the sample or standard.,

    Précision du teste

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level POTE Ankyrin Domain Family, Member G (POTEG) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level POTE Ankyrin Domain Family, Member G (POTEG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Conseil sur la manipulation

    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

    Date de péremption

    4-8 months
  • Antigène Tous les produits POTEG

    POTEG (POTE Ankyrin Domain Family, Member G (POTEG))

    Autre désignation

    POTE Ankyrin Domain Family, Member G

    Sujet

    Gene Name: POTE Ankyrin Domain Family, Member G

    Gene Aliases: A26C2, POTE14, POTE14a, CT104.4, ANKRD26-Like Family C Member 2, Cancer/Testis Antigen Family 104,Member 4, Prostate, ovary, testis-expressed protein on chromosome 14

    ID gène

    404785

    UniProt

    Q6S5H5
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