PROC Kit ELISA

N° du produit ABIN5658189

Détail du produit PROC Kit ELISA

Antigène: PROC (Vitamin K-dependent protein C (PROC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Type d'échantillon: Plasma

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Protein C (PROC). No significant cross-reactivity or interference between Protein C (PROC) and analogues was observed.

Sensibilité: 24.8 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein C (PROC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein C (PROC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein C (PROC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein C (PROC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein C (PROC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein C (PROC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PROC (Vitamin K-dependent protein C (PROC))

Autre désignation: Protein C (PROC Produits)

Synonymes: proc Kit ELISA, si:ch1073-188c16.3 Kit ELISA, zgc:63987 Kit ELISA, PROC Kit ELISA, APC Kit ELISA, PC Kit ELISA, PROC1 Kit ELISA, THPH3 Kit ELISA, THPH4 Kit ELISA, proc1 Kit ELISA, MGC64425 Kit ELISA, PA Kit ELISA, protein C, inactivator of coagulation factors Va and VIIIa Kit ELISA, protein C (inactivator of coagulation factors Va and VIIIa), a Kit ELISA, protein C (inactivator of coagulation factors Va and VIIIa) Kit ELISA, protein C, inactivator of coagulation factors Va and VIIIa S homeolog Kit ELISA, vitamin K-dependent protein C Kit ELISA, prosaposin Kit ELISA, protein C Kit ELISA, proline rich protein HaeIII subfamily 1 Kit ELISA, PROC Kit ELISA, proca Kit ELISA, proc.S Kit ELISA, proc Kit ELISA, CpipJ_CPIJ000393 Kit ELISA, CpipJ_CPIJ002754 Kit ELISA, CpipJ_CPIJ003717 Kit ELISA, CpipJ_CPIJ003718 Kit ELISA, CpipJ_CPIJ014440 Kit ELISA, CpipJ_CPIJ018032 Kit ELISA, CpipJ_CPIJ018737 Kit ELISA, PSAP Kit ELISA, Proc Kit ELISA, PRH1 Kit ELISA

Classe de substances: Viral Protein

Sujet:

Gene Name: Protein C

Gene Aliases: PRO-C, VKDPC, PC, Vitamin K Dependent Protein C, Inactivator Of Coagulation Factors Va And VIIIa, Autoprothrombin IIA, Blood coagulation factor XIV, Activation peptide

ID gène: 5624

UniProt: P04070