PDIA3 Kit ELISA

N° du produit ABIN5658201

Détail du produit PDIA3 Kit ELISA

Antigène: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A3 (PDIA3). No significant cross-reactivity or interference between Protein Disulfide Isomerase A3 (PDIA3) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A3 (PDIA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A3 (PDIA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A3 (PDIA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A3 (PDIA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase A3 (PDIA3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase A3 (PDIA3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Autre désignation: Protein Disulfide Isomerase A3 (PDIA3 Produits)

Synonymes: ER60 Kit ELISA, ERp57 Kit ELISA, ERp60 Kit ELISA, ERp61 Kit ELISA, GRP57 Kit ELISA, GRP58 Kit ELISA, HsT17083 Kit ELISA, P58 Kit ELISA, PI-PLC Kit ELISA, grp-58 Kit ELISA, 58kDa Kit ELISA, Erp Kit ELISA, Grp58 Kit ELISA, PDI Kit ELISA, PDI-Q2 Kit ELISA, PLC[a] Kit ELISA, Plca Kit ELISA, 1 Kit ELISA, 25D3-membrane-associated Kit ELISA, sb:cb825 Kit ELISA, erp57 Kit ELISA, grp58 Kit ELISA, pdia3 Kit ELISA, protein disulfide isomerase family A member 3 Kit ELISA, protein disulfide-isomerase A3 Kit ELISA, protein disulfide isomerase associated 3 Kit ELISA, protein disulfide isomerase family A, member 3 Kit ELISA, protein disulfide isomerase family A member 3 S homeolog Kit ELISA, PDIA3 Kit ELISA, Tsp_10062 Kit ELISA, Pdia3 Kit ELISA, pdia3 Kit ELISA, pdia3.S Kit ELISA

Sujet:

Gene Name: Protein Disulfide Isomerase A3

Gene Aliases: P58, ERp61, ERp57, ERp60, GRP57, PI-PLC, GRP58, Glucose Regulated Protein,58 kDa, Protein Disulfide Isomerase-Associated 3, Endoplasmic reticulum resident protein 57

ID gène: 2923

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis