PKN1 Kit ELISA

N° du produit ABIN5658228

Détail du produit PKN1 Kit ELISA

Antigène: PKN1 (Protein Kinase N1 (PKN1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Protein Kinase N1 (PKN1). No significant cross-reactivity or interference between Protein Kinase N1 (PKN1) and analogues was observed.

Sensibilité: 0.117 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase N1 (PKN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase N1 (PKN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase N1 (PKN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase N1 (PKN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase N1 (PKN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase N1 (PKN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PKN1 (Protein Kinase N1 (PKN1))

Autre désignation: Protein Kinase N1 (PKN1 Produits)

Synonymes: DBK Kit ELISA, PAK-1 Kit ELISA, PAK1 Kit ELISA, PKN Kit ELISA, PKN-ALPHA Kit ELISA, PRK1 Kit ELISA, PRKCL1 Kit ELISA, F730027O18Rik Kit ELISA, Pkn Kit ELISA, Prkcl1 Kit ELISA, Stk3 Kit ELISA, PKN1 Kit ELISA, 3-2 Kit ELISA, CG2049 Kit ELISA, CG2055 Kit ELISA, CT6637 Kit ELISA, CT6660 Kit ELISA, DPKN Kit ELISA, Dmel\\CG2049 Kit ELISA, Dpkn Kit ELISA, PRK2 Kit ELISA, Pk45C Kit ELISA, Pk?3 Kit ELISA, dPKN Kit ELISA, l(2)06736 Kit ELISA, l(2)45Ca Kit ELISA, v(2)rG232 Kit ELISA, protein kinase N1 Kit ELISA, protein kinase N1b Kit ELISA, Protein kinase N Kit ELISA, PKN1 Kit ELISA, Pkn1 Kit ELISA, pkn1b Kit ELISA, pkn1 Kit ELISA, Pkn Kit ELISA

Sujet:

Gene Name: Protein Kinase N1

Gene Aliases: PAK1, DBK, PRK1, PRKCL1, Protein Kinase C-Like 1, Protein kinase PKN-alpha, Protein-kinase C-related kinase 1, Serine-threonine protein kinase N

ID gène: 5585

UniProt: Q16512