PLP1 Kit ELISA

N° du produit ABIN5658278

Kit ELISA PLP1 Humain, Colorimetric test pour la quantification de Humain PLP1.

Aperçu rapide pour PLP1 Kit ELISA (ABIN5658278)

Antigène: PLP1 (Proteolipid Protein 1 (PLP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12 ng/mL - 200 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit PLP1 Kit ELISA

Seuil minimal de détection: 3.12 ng/mL

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Proteolipid Protein 1, Myelin (PLP1). No significant cross-reactivity or interference between Proteolipid Protein 1, Myelin (PLP1) and analogues was observed.

Sensibilité: 1.12 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Proteolipid Protein 1, Myelin (PLP1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Proteolipid Protein 1, Myelin (PLP1) and unlabeled Proteolipid Protein 1, Myelin (PLP1) (Standards or samples) with the pre-coated antibody specific to Proteolipid Protein 1, Myelin (PLP1). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Proteolipid Protein 1, Myelin (PLP1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Proteolipid Protein 1, Myelin (PLP1) in the sample.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proteolipid Protein 1, Myelin (PLP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proteolipid Protein 1, Myelin (PLP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PLP1 (Proteolipid Protein 1 (PLP1))

Autre désignation: Proteolipid Protein 1, Myelin

Sujet:

Gene Name: Proteolipid Protein 1, Myelin

Gene Aliases: MMPL, PLP/DM20, PMD, SPG2, Pelizaeus-Merzbacher Disease, Spastic Paraplegia 2,Uncomplicated, Myelin proteolipid protein, Lipophilin