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PKLR Kit ELISA

PKLR Reactivité: Humain Colorimetric Sandwich ELISA 39.06 pg/mL - 2500 pg/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5658320
  • Antigène Voir toutes PKLR Kits ELISA
    PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
    Reactivité
    • 2
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    39.06 pg/mL - 2500 pg/mL
    Seuil minimal de détection
    39.06 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Pyruvate Kinase, Liver And RBC (PK). No significant cross-reactivity or interference between Pyruvate Kinase, Liver And RBC (PK) and analogues was observed.
    Sensibilité
    17 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Kinase, Liver And RBC (PK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Kinase, Liver And RBC (PK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Kinase, Liver And RBC (PK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Kinase, Liver And RBC (PK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Kinase, Liver And RBC (PK) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Kinase, Liver And RBC (PK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes PKLR Kits ELISA
    PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
    Autre désignation
    Pyruvate Kinase, Liver And RBC (PKLR Produits)
    Synonymes
    PK1 Kit ELISA, PKL Kit ELISA, PKR Kit ELISA, PKRL Kit ELISA, RPK Kit ELISA, Pklg Kit ELISA, wu:fd15e01 Kit ELISA, wu:fi37e08 Kit ELISA, pk1 Kit ELISA, PKLR Kit ELISA, Pk-1 Kit ELISA, Pk1 Kit ELISA, R-PK Kit ELISA, pklr Kit ELISA, pyruvate kinase L/R Kit ELISA, pyruvate kinase, liver and RBC Kit ELISA, pyruvate kinase, liver and RBC L homeolog Kit ELISA, pyruvate kinase liver and red blood cell Kit ELISA, pyruvate kinase PKLR-like Kit ELISA, PKLR Kit ELISA, Pklr Kit ELISA, pklr Kit ELISA, pklr.L Kit ELISA, LOC100621940 Kit ELISA
    Sujet

    Gene Name: Pyruvate Kinase, Liver And RBC

    Gene Aliases: PKLR, PyK, PK1, PKL, Pyruvate Kinase Isozymes R/L, Glutamic--pyruvic transaminase 1, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase

    ID gène
    5313
    UniProt
    P30613
    Pathways
    Ribonucleoside Biosynthetic Process
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