QSOX1 Kit ELISA

N° du produit ABIN5658325

Détail du produit QSOX1 Kit ELISA

Antigène: QSOX1 (Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1). No significant cross-reactivity or interference between Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) and analogues was observed.

Sensibilité: 0.131 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: QSOX1 (Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1))

Autre désignation: Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1 Produits)

Synonymes: qscn6l1 Kit ELISA, Qscn6 Kit ELISA, Qsox Kit ELISA, Sox-2 Kit ELISA, rSOx Kit ELISA, Q6 Kit ELISA, QSCN6 Kit ELISA, 1300003H02Rik Kit ELISA, QSOX Kit ELISA, SOx Kit ELISA, GEC-3 Kit ELISA, GEC3 Kit ELISA, SOX3 Kit ELISA, ATQSOX1 Kit ELISA, QSO2 Kit ELISA, T15D22.7 Kit ELISA, T15D22_7 Kit ELISA, quiescin-sulfhydryl oxidase 1 Kit ELISA, quiescin sulfhydryl oxidase 1 Kit ELISA, quiescin sulfhydryl oxidase 1 L homeolog Kit ELISA, quiescin Q6 sulfhydryl oxidase 1 Kit ELISA, quiescin-sulfhydryl oxidase 1 Kit ELISA, QSOX1 Kit ELISA, qsox1 Kit ELISA, qsox1.L Kit ELISA, Qsox1 Kit ELISA

Sujet:

Gene Name: Quiescin Q6 Sulfhydryl Oxidase 1

Gene Aliases: Q6, QSCN6

ID gène: 5768

UniProt: O00391

Pathways: Cell RedoxHomeostasis