QDPR Kit ELISA

N° du produit ABIN5658327

Détail du produit QDPR Kit ELISA

Antigène: QDPR (Quinoid Dihydropteridine Reductase (QDPR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Quinoid Dihydropteridine Reductase (QDPR). No significant cross-reactivity or interference between Quinoid Dihydropteridine Reductase (QDPR) and analogues was observed.

Sensibilité: 0.051 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Quinoid Dihydropteridine Reductase (QDPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Quinoid Dihydropteridine Reductase (QDPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Quinoid Dihydropteridine Reductase (QDPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Quinoid Dihydropteridine Reductase (QDPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Quinoid Dihydropteridine Reductase (QDPR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Quinoid Dihydropteridine Reductase (QDPR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: QDPR (Quinoid Dihydropteridine Reductase (QDPR))

Autre désignation: Quinoid Dihydropteridine Reductase (QDPR Produits)

Synonymes: DHPR Kit ELISA, PKU2 Kit ELISA, SDR33C1 Kit ELISA, 2610008L04Rik Kit ELISA, D5Ertd371e Kit ELISA, Dhpr Kit ELISA, QDPR Kit ELISA, qdpr Kit ELISA, MGC131372 Kit ELISA, DDBDRAFT_0168923 Kit ELISA, DDBDRAFT_0237752 Kit ELISA, DDB_0168923 Kit ELISA, DDB_0237752 Kit ELISA, CG4665 Kit ELISA, Dmel\\CG4665 Kit ELISA, si:ch211-89f22.2 Kit ELISA, quinoid dihydropteridine reductase Kit ELISA, quinoid dihydropteridine reductase S homeolog Kit ELISA, dihydropteridine reductase Kit ELISA, Dihydropteridine reductase Kit ELISA, bifunctional oxygen-insensitive NAD(P)H nitroreductase/dihydropteridine reductase NfnB Kit ELISA, quinoid dihydropteridine reductase a Kit ELISA, QDPR Kit ELISA, Qdpr Kit ELISA, qdpr Kit ELISA, qdpr.S Kit ELISA, Dhpr Kit ELISA, nfnB Kit ELISA, LOC5568428 Kit ELISA, dhpr Kit ELISA, qdpra Kit ELISA

Sujet:

Gene Name: Quinoid Dihydropteridine Reductase

Gene Aliases: DHPR, PKU2, SDR33C1, HDHPR, 6,7-Dihydropteridine Reductase, Short Chain Dehydrogenase/Reductase Family 33C,Member 1

ID gène: 5860

UniProt: P09417