RAB5 Kit ELISA

N° du produit ABIN5658343

Détail du produit RAB5 Kit ELISA

Antigène: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of RAB5A, Member RAS Oncogene Family (RAB5A). No significant cross-reactivity or interference between RAB5A, Member RAS Oncogene Family (RAB5A) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAB5A, Member RAS Oncogene Family (RAB5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAB5A, Member RAS Oncogene Family (RAB5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Autre désignation: RAB5A, Member RAS Oncogene Family (RAB5A Produits)

Synonymes: RAB5 Kit ELISA, 2410015H04Rik Kit ELISA, AI663973 Kit ELISA, AU021172 Kit ELISA, nnyRab5a Kit ELISA, DDBDRAFT_0168594 Kit ELISA, DDBDRAFT_0229401 Kit ELISA, DDB_0168594 Kit ELISA, DDB_0229401 Kit ELISA, rab5 Kit ELISA, RAB5A Kit ELISA, Rab5a Kit ELISA, rab5al Kit ELISA, zgc:56644 Kit ELISA, fj38g08 Kit ELISA, hm:zehn1144 Kit ELISA, rab5a Kit ELISA, wu:fj38g08 Kit ELISA, RAB5A, member RAS oncogene family Kit ELISA, rab5A protein Kit ELISA, Rab5a, GTPase Kit ELISA, predicted protein Kit ELISA, Rab GTPase Kit ELISA, RAB5A, member RAS oncogene family L homeolog Kit ELISA, RAB5A, member RAS oncogene family, b Kit ELISA, RAB5A, member RAS oncogene family, a Kit ELISA, RAB5A Kit ELISA, Rab5a Kit ELISA, rab5A Kit ELISA, rab5a Kit ELISA, rab5a.L Kit ELISA, rab5ab Kit ELISA, rab5aa Kit ELISA

Sujet:

Gene Name: RAB5A, Member RAS Oncogene Family

Gene Aliases: RAB5

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity