RORA Kit ELISA

N° du produit ABIN5658351

Détail du produit RORA Kit ELISA

Antigène: RORA (RAR-Related Orphan Receptor A (RORA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of RAR Related Orphan Receptor Alpha (RORa). No significant cross-reactivity or interference between RAR Related Orphan Receptor Alpha (RORa) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAR Related Orphan Receptor Alpha (RORa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAR Related Orphan Receptor Alpha (RORa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAR Related Orphan Receptor Alpha (RORa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAR Related Orphan Receptor Alpha (RORa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAR Related Orphan Receptor Alpha (RORa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAR Related Orphan Receptor Alpha (RORa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RORA (RAR-Related Orphan Receptor A (RORA))

Autre désignation: RAR Related Orphan Receptor Alpha (RORA Produits)

Synonymes: ror1 Kit ELISA, ror2 Kit ELISA, ror3 Kit ELISA, rzra Kit ELISA, nr1f1 Kit ELISA, MGC146531 Kit ELISA, NR1F1 Kit ELISA, ROR1 Kit ELISA, ROR2 Kit ELISA, ROR3 Kit ELISA, RZR-ALPHA Kit ELISA, RZRA Kit ELISA, RORalpha-B Kit ELISA, gb:dq017624 Kit ELISA, rora2 Kit ELISA, 9530021D13Rik Kit ELISA, Nr1f1 Kit ELISA, nmf267 Kit ELISA, sg Kit ELISA, staggerer Kit ELISA, tmgc26 Kit ELISA, RORalpha1 Kit ELISA, RAR related orphan receptor A Kit ELISA, RAR-related orphan receptor A Kit ELISA, RAR-related orphan receptor A, paralog a Kit ELISA, RAR-related orphan receptor alpha Kit ELISA, RORA Kit ELISA, rora Kit ELISA, Rora Kit ELISA, roraa Kit ELISA

Sujet:

Gene Name: RAR Related Orphan Receptor Alpha

Gene Aliases: ROR1, ROR2, NR1F1, ROR3, RZRA, Nuclear receptor subfamily 1 group F member 1, Retinoid-related orphan receptor-alpha

ID gène: 6095

UniProt: P35398

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha