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Retinoic Acid Receptor beta Kit ELISA

RARB Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5658466
  • Antigène Voir toutes Retinoic Acid Receptor beta (RARB) Kits ELISA
    Retinoic Acid Receptor beta (RARB) (Retinoic Acid Receptor, beta (RARB))
    Reactivité
    • 4
    • 3
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Retinoic Acid Receptor Beta (RARb). No significant cross-reactivity or interference between Retinoic Acid Receptor Beta (RARb) and analogues was observed.
    Sensibilité
    0.054 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoic Acid Receptor Beta (RARb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoic Acid Receptor Beta (RARb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoic Acid Receptor Beta (RARb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinoic Acid Receptor Beta (RARb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinoic Acid Receptor Beta (RARb) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinoic Acid Receptor Beta (RARb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Retinoic Acid Receptor beta (RARB) Kits ELISA
    Retinoic Acid Receptor beta (RARB) (Retinoic Acid Receptor, beta (RARB))
    Autre désignation
    Retinoic Acid Receptor Beta (RARB Produits)
    Synonymes
    RARB Kit ELISA, nRARs Kit ELISA, HAP Kit ELISA, NR1B2 Kit ELISA, RRB2 Kit ELISA, A830025K23 Kit ELISA, Hap Kit ELISA, Nr1b2 Kit ELISA, RARbeta2 Kit ELISA, RARBETA Kit ELISA, retinoic acid receptor beta Kit ELISA, retinoic acid receptor, beta Kit ELISA, RARB Kit ELISA, Rarb Kit ELISA
    Sujet

    Gene Name: Retinoic Acid Receptor Beta

    Gene Aliases: HAP, NR1B2, RRB2, RAR-epsilon, HBV-activated protein, Nuclear receptor subfamily 1 group B member 2

    ID gène
    5915
    UniProt
    P10826
    Pathways
    Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
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