RUNX2 Kit ELISA

N° du produit ABIN5658532

Détail du produit RUNX2 Kit ELISA

Antigène: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Runt Related Transcription Factor 2 (RUNX2). No significant cross-reactivity or interference between Runt Related Transcription Factor 2 (RUNX2) and analogues was observed.

Sensibilité: 0.116 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Runt Related Transcription Factor 2 (RUNX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Runt Related Transcription Factor 2 (RUNX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Runt Related Transcription Factor 2 (RUNX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Runt Related Transcription Factor 2 (RUNX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Runt Related Transcription Factor 2 (RUNX2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Runt Related Transcription Factor 2 (RUNX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Autre désignation: Runt Related Transcription Factor 2 (RUNX2 Produits)

Synonymes: AML3 Kit ELISA, CBF-alpha-1 Kit ELISA, CBFA1 Kit ELISA, CCD Kit ELISA, CCD1 Kit ELISA, CLCD Kit ELISA, OSF-2 Kit ELISA, OSF2 Kit ELISA, PEA2aA Kit ELISA, PEBP2aA Kit ELISA, Cbf Kit ELISA, Cbfa-1 Kit ELISA, Cbfa1 Kit ELISA, LS3 Kit ELISA, Osf2 Kit ELISA, Pebp2a1 Kit ELISA, Pebpa2a Kit ELISA, runx2 Kit ELISA, RUNX2 Kit ELISA, ccd Kit ELISA, aml3 Kit ELISA, ccd1 Kit ELISA, osf2 Kit ELISA, cbfa1 Kit ELISA, pea2aa Kit ELISA, pebp2a1 Kit ELISA, pebp2a2 Kit ELISA, pebp2aa Kit ELISA, pebp2aa1 Kit ELISA, runt related transcription factor 2 Kit ELISA, runt-related transcription factor 2a Kit ELISA, runt-related transcription factor 2 Kit ELISA, runt related transcription factor 2 L homeolog Kit ELISA, RUNX2 Kit ELISA, runx2a Kit ELISA, Runx2 Kit ELISA, runx2 Kit ELISA, LOC703331 Kit ELISA, LOC100549663 Kit ELISA, runx2.L Kit ELISA

Sujet:

Gene Name: Runt Related Transcription Factor 2

Gene Aliases: AML3, CBFA1, CCD1, OSF2, PEBP2A, Acute myeloid leukemia 3 , Core-binding factor alpha-1, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 A

ID gène: 12393

UniProt: Q08775