SIK2 Kit ELISA

N° du produit ABIN5658588

Détail du produit SIK2 Kit ELISA

Antigène: SIK2 (Salt-Inducible Kinase 2 (SIK2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Salt Inducible Kinase 2 (SIK2). No significant cross-reactivity or interference between Salt Inducible Kinase 2 (SIK2) and analogues was observed.

Sensibilité: 0.065 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Salt Inducible Kinase 2 (SIK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Salt Inducible Kinase 2 (SIK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Salt Inducible Kinase 2 (SIK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Salt Inducible Kinase 2 (SIK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Salt Inducible Kinase 2 (SIK2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Salt Inducible Kinase 2 (SIK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SIK2 (Salt-Inducible Kinase 2 (SIK2))

Autre désignation: Salt Inducible Kinase 2 (SIK2 Produits)

Synonymes: PIG33 Kit ELISA, SC1 Kit ELISA, LOH11CR1I Kit ELISA, QIK Kit ELISA, SNF1LK2 Kit ELISA, G630080D20Rik Kit ELISA, Snf1lk2 Kit ELISA, RGD1559698 Kit ELISA, si:dkey-27p23.1 Kit ELISA, snf1lk2a Kit ELISA, SPARC like 1 Kit ELISA, salt inducible kinase 2 Kit ELISA, salt-inducible kinase 2 Kit ELISA, salt-inducible kinase 2a Kit ELISA, SPARCL1 Kit ELISA, SIK2 Kit ELISA, SLK2 Kit ELISA, Sik2 Kit ELISA, sik2a Kit ELISA

Sujet:

Gene Name: Salt Inducible Kinase 2

Gene Aliases: LOH11CR1I, QIK, SNF1LK2, SNF1-Like Kinase 2, Qin-induced kinase, Serine/threonine-protein kinase SNF1-like kinase 2

ID gène: 23235

UniProt: Q9H0K1