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SGCD Kit ELISA

SGCD Reactivité: Humain Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
N° du produit ABIN5658595
  • Antigène Voir toutes SGCD Kits ELISA
    SGCD (Sarcoglycan, delta (35kDa Dystrophin-Associated Glycoprotein) (SGCD))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.781 ng/mL - 50 ng/mL
    Seuil minimal de détection
    0.781 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Sarcoglycan Delta (SGCd). No significant cross-reactivity or interference between Sarcoglycan Delta (SGCd) and analogues was observed.
    Sensibilité
    0.28 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sarcoglycan Delta (SGCd). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sarcoglycan Delta (SGCd). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sarcoglycan Delta (SGCd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sarcoglycan Delta (SGCd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sarcoglycan Delta (SGCd) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sarcoglycan Delta (SGCd) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes SGCD Kits ELISA
    SGCD (Sarcoglycan, delta (35kDa Dystrophin-Associated Glycoprotein) (SGCD))
    Autre désignation
    Sarcoglycan Delta (SGCD Produits)
    Synonymes
    BEST:GH21860 Kit ELISA, CG14808 Kit ELISA, CT34621 Kit ELISA, DmSCG-gammadelta Kit ELISA, Dmel\\CG14808 Kit ELISA, EG:4F1.1 Kit ELISA, GH21860 Kit ELISA, Sgcd Kit ELISA, wu:fc49e11 Kit ELISA, zgc:110649 Kit ELISA, 35DAG Kit ELISA, CMD1L Kit ELISA, DAGD Kit ELISA, SG-delta Kit ELISA, SGCDP Kit ELISA, SGD Kit ELISA, Delta-SG Kit ELISA, 35kDa Kit ELISA, delta-SG Kit ELISA, sarcoglycan delta Kit ELISA, Sarcoglycan delta Kit ELISA, sarcoglycan, delta (dystrophin-associated glycoprotein) Kit ELISA, sarcoglycan, delta Kit ELISA, SGCD Kit ELISA, Scgdelta Kit ELISA, sgcd Kit ELISA, Sgcd Kit ELISA
    Sujet

    Gene Name: Sarcoglycan Delta

    Gene Aliases: 35DAG, CMD1L, DAGD, SG-delta, SGCDP, SGD, 35 kDa Dystrophin-Associated Glycoprotein

    ID gène
    6444
    UniProt
    Q92629
    Pathways
    Maintenance of Protein Location
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