SOX9 Kit ELISA

N° du produit ABIN5658768

Détail du produit SOX9 Kit ELISA

Antigène: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Sex Determining Region Y Box Protein 9 (SOX9). No significant cross-reactivity or interference between Sex Determining Region Y Box Protein 9 (SOX9) and analogues was observed.

Sensibilité: 0.129 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sex Determining Region Y Box Protein 9 (SOX9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sex Determining Region Y Box Protein 9 (SOX9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sex Determining Region Y Box Protein 9 (SOX9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sex Determining Region Y Box Protein 9 (SOX9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sex Determining Region Y Box Protein 9 (SOX9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sex Determining Region Y Box Protein 9 (SOX9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Autre désignation: Sex Determining Region Y Box Protein 9 (SOX9 Produits)

Synonymes: CMD1 Kit ELISA, CMPD1 Kit ELISA, SRA1 Kit ELISA, LOC100227849 Kit ELISA, 2010306G03Rik Kit ELISA, AV220920 Kit ELISA, mKIAA4243 Kit ELISA, SOX-9 Kit ELISA, cmd1 Kit ELISA, cmpd1 Kit ELISA, sox-9 Kit ELISA, sox9 Kit ELISA, sox9-a Kit ELISA, sox9-b Kit ELISA, sox9b Kit ELISA, sra1 Kit ELISA, SRY-box 9 Kit ELISA, transcription factor SOX-9 Kit ELISA, SRY (sex determining region Y)-box 9 Kit ELISA, SRY box 9 Kit ELISA, SRY-box 9 L homeolog Kit ELISA, SOX9 Kit ELISA, LOC100227849 Kit ELISA, Sox9 Kit ELISA, sox9.L Kit ELISA

Sujet:

Gene Name: Sex Determining Region Y Box Protein 9

Gene Aliases: CMPD1, CMD1, SRA1, Campomelic Dysplasia,Autosomal Sex-Reversal, Transcription factor SOX-9

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development