SF3B3 Kit ELISA

N° du produit ABIN5658926

Détail du produit SF3B3 Kit ELISA

Antigène: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Splicing Factor 3B Subunit 3 (SF3B3). No significant cross-reactivity or interference between Splicing Factor 3B Subunit 3 (SF3B3) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Splicing Factor 3B Subunit 3 (SF3B3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Splicing Factor 3B Subunit 3 (SF3B3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Splicing Factor 3B Subunit 3 (SF3B3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Splicing Factor 3B Subunit 3 (SF3B3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Splicing Factor 3B Subunit 3 (SF3B3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Splicing Factor 3B Subunit 3 (SF3B3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Autre désignation: Splicing Factor 3B Subunit 3 (SF3B3 Produits)

Synonymes: GB11574 Kit ELISA, rse1 Kit ELISA, sap130 Kit ELISA, sf3b130 Kit ELISA, staf130 Kit ELISA, sf3b3 Kit ELISA, 1810061H24Rik Kit ELISA, 5730409A01Rik Kit ELISA, AA409318 Kit ELISA, D8Ertd633e Kit ELISA, RSE1 Kit ELISA, SAP130 Kit ELISA, mKIAA0017 Kit ELISA, ik:tdsubc_2b2 Kit ELISA, wu:fb81f05 Kit ELISA, xx:tdsubc_2b2 Kit ELISA, zgc:55440 Kit ELISA, SF3b130 Kit ELISA, STAF130 Kit ELISA, splicing factor 3b subunit 3 Kit ELISA, splicing factor 3B subunit 3 Kit ELISA, splicing factor 3b, subunit 3, 130kDa Kit ELISA, splicing factor 3b subunit 3 L homeolog Kit ELISA, splicing factor 3b, subunit 3 Kit ELISA, SF3B3 Kit ELISA, LOC550935 Kit ELISA, sf3b3 Kit ELISA, Sf3b3 Kit ELISA, LOC100178056 Kit ELISA, sf3b3.L Kit ELISA

Sujet:

Gene Name: Splicing Factor 3B Subunit 3

Gene Aliases: RSE1, SAP130, SF3b130, STAF130, Spliceosome-associated protein 130, Pre-mRNA-splicing factor SF3b 130 kDa subunit

ID gène: 101943

UniProt: Q921M3