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SPON2 Kit ELISA

SPON2 Reactivité: Souris Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5658929
  • Antigène Voir toutes SPON2 Kits ELISA
    SPON2 (Spondin 2 (SPON2))
    Reactivité
    • 2
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Spondin 2 (SPON2). No significant cross-reactivity or interference between Spondin 2 (SPON2) and analogues was observed.
    Sensibilité
    0.058 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spondin 2 (SPON2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spondin 2 (SPON2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spondin 2 (SPON2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Spondin 2 (SPON2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Spondin 2 (SPON2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Spondin 2 (SPON2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes SPON2 Kits ELISA
    SPON2 (Spondin 2 (SPON2))
    Autre désignation
    Spondin 2 (SPON2 Produits)
    Synonymes
    DIL-1 Kit ELISA, DIL1 Kit ELISA, M-SPONDIN Kit ELISA, MINDIN Kit ELISA, 2310045I24Rik Kit ELISA, AI504350 Kit ELISA, M-spondin Kit ELISA, Mindin Kit ELISA, Mspondin Kit ELISA, etID309958.14 Kit ELISA, mindin2 Kit ELISA, zgc:100756 Kit ELISA, mindin1 Kit ELISA, spondin 2 Kit ELISA, spondin 2, extracellular matrix protein Kit ELISA, spondin 2, extracellular matrix protein S homeolog Kit ELISA, spondin 2b, extracellular matrix protein Kit ELISA, spondin 2a, extracellular matrix protein Kit ELISA, SPON2 Kit ELISA, Spon2 Kit ELISA, spon2.S Kit ELISA, spon2b Kit ELISA, spon2a Kit ELISA
    Sujet

    Gene Name: Spondin 2

    Gene Aliases: DIL1, Mindin, M-spondin, Extracellular Matrix Protein, Differentially expressed in cancerous and non-cancerous lung cells 1

    ID gène
    100689
    UniProt
    Q8BMS2
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