STIP1 Kit ELISA

N° du produit ABIN5658966

Détail du produit STIP1 Kit ELISA

Antigène: STIP1 (Stress-Induced-phosphoprotein 1 (STIP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Stress Induced Phosphoprotein 1 (STIP1). No significant cross-reactivity or interference between Stress Induced Phosphoprotein 1 (STIP1) and analogues was observed.

Sensibilité: 0.63 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stress Induced Phosphoprotein 1 (STIP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stress Induced Phosphoprotein 1 (STIP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stress Induced Phosphoprotein 1 (STIP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stress Induced Phosphoprotein 1 (STIP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stress Induced Phosphoprotein 1 (STIP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stress Induced Phosphoprotein 1 (STIP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: STIP1 (Stress-Induced-phosphoprotein 1 (STIP1))

Autre désignation: Stress Induced Phosphoprotein 1 (STIP1 Produits)

Synonymes: HOP Kit ELISA, IEF-SSP-3521 Kit ELISA, P60 Kit ELISA, STI1 Kit ELISA, STI1L Kit ELISA, Hop Kit ELISA, Sti1 Kit ELISA, p60 Kit ELISA, MGC53256 Kit ELISA, stip1 Kit ELISA, MGC76181 Kit ELISA, MGC82554 Kit ELISA, zgc:92133 Kit ELISA, stress induced phosphoprotein 1 Kit ELISA, stress-induced phosphoprotein 1 Kit ELISA, stress induced phosphoprotein 1 L homeolog Kit ELISA, Stress-induced-phosphoprotein 1 Kit ELISA, stress-induced-phosphoprotein 1 Kit ELISA, stress induced phosphoprotein 1 S homeolog Kit ELISA, STIP1 Kit ELISA, Stip1 Kit ELISA, stip1.L Kit ELISA, GL50803_27310 Kit ELISA, PGTG_06468 Kit ELISA, stip1 Kit ELISA, stip1.S Kit ELISA

Sujet:

Gene Name: Stress Induced Phosphoprotein 1

Gene Aliases: HOP, P60, IEF-SSP-3521, STI1L, Hsp70/Hsp90-Organizing Protein, Renal carcinoma antigen NY-REN-11, Transformation-sensitive protein IEF SSP 3521

ID gène: 10963

UniProt: P31948