SNAP25 Kit ELISA

N° du produit ABIN5659053

Détail du produit SNAP25 Kit ELISA

Antigène: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Lapin, Porc, Cheval

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Synaptosomal Associated Protein 25 kDa (SNAP25). No significant cross-reactivity or interference between Synaptosomal Associated Protein 25 kDa (SNAP25) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synaptosomal Associated Protein 25 kDa (SNAP25). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synaptosomal Associated Protein 25 kDa (SNAP25). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synaptosomal Associated Protein 25 kDa (SNAP25), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Synaptosomal Associated Protein 25 kDa (SNAP25) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synaptosomal Associated Protein 25kDa (SNAP25) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synaptosomal Associated Protein 25kDa (SNAP25) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Autre désignation: Synaptosomal Associated Protein 25kDa (SNAP25 Produits)

Synonymes: RIC-4 Kit ELISA, RIC4 Kit ELISA, SEC9 Kit ELISA, SNAP Kit ELISA, SNAP-25 Kit ELISA, bA416N4.2 Kit ELISA, dJ1068F16.2 Kit ELISA, SNAP-25B Kit ELISA, SNAP-25a Kit ELISA, ric-4 Kit ELISA, ric4 Kit ELISA, sec9 Kit ELISA, snap-25 Kit ELISA, Bdr Kit ELISA, sp Kit ELISA, fj55h11 Kit ELISA, snap25 Kit ELISA, wu:fj55h11 Kit ELISA, Snap Kit ELISA, snap25.2 Kit ELISA, wu:fj33h05 Kit ELISA, synaptosome associated protein 25 Kit ELISA, synaptosome associated protein 25kDa S homeolog Kit ELISA, synaptosomal-associated protein, 25kDa Kit ELISA, synaptosomal-associated protein 25 Kit ELISA, synaptosome associated protein 25kDa Kit ELISA, synaptosome associated protein 25kDa L homeolog Kit ELISA, zgc:101731 Kit ELISA, synaptosomal-associated protein, 25b Kit ELISA, SNAP25 Kit ELISA, Snap25 Kit ELISA, snap25.S Kit ELISA, snap25 Kit ELISA, BACOIKO003_07 Kit ELISA, Tsp_10811 Kit ELISA, snap25.L Kit ELISA, zgc:101731 Kit ELISA, snap25b Kit ELISA

Sujet:

Gene Name: Synaptosomal Associated Protein 25 kDa

Gene Aliases: RIC4, SEC9, SNAP, SUP, Super protein, Resistance To Inhibitors Of Cholinesterase 4 Homolog

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport